Genetic linkage map in sour cherry using RFLP markers

Restriction fragment length polymorphism (RFLP) linkage maps of two tetrapl oid sour cherry (Prunus cerasus L., 2n = 4x = 32) cultivars, Rheinische Sch attenmorelle (RS) and Erdi Botermo (EB), were constructed from 86 progeny f rom the cross RS x EB. The RS linkage map consists of 126 single-dose restr iction fragment (SDRF, Wu et al. 1992) markers assigned to 19 linkage group s covering 461.6 cM. The EB linkage map has 95 SDRF markers assigned to 16 linkage groups covering 279.2 cM. Fifty three markers mapped in both parent s were used as bridges between both maps and 13 sets of homologous linkage groups were identified. Homoeologous relationships among the sour cherry li nkage groups could not be determined because only 15 probes identified dupl icate loci. Fifty nine of the markers on the linkage maps were detected wit h probes used in other Prunus genetic linkage maps. Four of the sour cherry linkage groups may be homologous with four of the eight genetic linkage gr oups identified in peach and almond. Twenty one fragments expected to segre gate in a 1 : 1 ratio segregated in a 2 : 1 ratio. Three of these fragments were used in the final map construction because they all mapped to the sam e linkage group. Six fragments exhibited segregation consistent with the ex pectations of intergenomic pairing and/or recombination.