Lh. Bruner et al., Investigation of ingredient interactions in cosmetic formulations using isolated bovine corneas, TOX VITRO, 12(6), 1998, pp. 669-690
The purpose of this paper is to report on use of a modified bovine cornea o
pacity and permeability assay (BCOP) to test the effects of several cosmeti
c formulations on eye-derived tissue in vitro. The results from these studi
es suggest that a BCOP protocol using prolonged exposure and repeated treat
ments may be useful for screening the eye effects of cosmetic formulations.
Further work will be required, however, before the model is ready for form
al validation. This series of experiments also provides an example of where
the toxicity of one ingredient was significantly changed by its interactio
n with other ingredients in a mixture. As it was not possible to predict th
e highly reactive nature of the formulation in vitro based on an evaluation
of ingredient toxicity data alone, this case illustrates the importance of
obtaining adequate safety testing data on innovative mixtures of cosmetic
ingredients before human exposure is allowed. (C) 1998 Elsevier Science Ltd
. All rights reserved.