S. Adkins et Cc. Kao, Subgenomic RNA promoters dictate the mode of recognition by bromoviral RNA-dependent RNA polymerases, VIROLOGY, 252(1), 1998, pp. 1-8
Both the brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV)
RNA-dependent RNA polymerases (RdRps) were found to recognize the BMV core
subgenomic promoter in the same manner, requiring specific functional group
s at positions -17, -14, -13, and -11 relative to the subgenomic initiation
site (fl). For CCMV subgenomic RNA synthesis, both RdRps required the same
nucleotides and four additional nucleotides at positions -20, -16, -15, an
d -10. The -20 nucleotide is partially responsible for the differential mod
e of recognition of the two promoters. These data provide evidence that the
RNA can induce RdRps to alter the mode of promoter recognition. (C) 1998 A
cademic Press.