Al. Giuliani et al., Rabbit IgG antibodies against cord red blood cell membranes bind to complement receptor 1 (CD35), ACT HAEMAT, 100(3), 1998, pp. 123-129
We have previously shown that a subpopulation of cord/fetal red blood cells
(RBC) binds rabbit IgG antibodies raised against cord RBC and absorbed on
adult RBC (F-IgG), while control IgG, raised against and absorbed on adult
RBC (A-IgG), fails to do so. In the present study, F-IgG maintained its bin
ding to cord RBC surface antigens following absorption on spectrin but not
after absorption on skeleton-stripped RBC membranes. Spectrin-absorbed F-Ig
G-but not A-IgG-affinity-purified material from cord RBC contained polypept
ides with apparent MW of complement receptor 1 (CR1) allotypes, Moreover, o
n immunoblotting these polypeptides reacted with I-125-F-IgG as well as wit
h I-125-anti-CR1 mAb, and binding of I-125-anti-CR1 mAb was inhibited by un
labelled F-IgG, In addition, cord RBC incubated with F-IgG prior to reactio
n with anti-CR1 showed decreased fluorescence intensity on flow cytometry,
Taken together the results suggest that F-IgG binds to CR1 which shows incr
eased expression/accessibility on a subpopulation of cord/fetal RBC.