EFFICIENT DETECTION OF DNA POLYMORPHISMS BY FLUORESCENT RAPD ANALYSIS

A method is presented for the analysis of fluorescently labeled random amplified polymorphic DNA (FRAPD) fragments. A DNA sequencer and coll ection and analysis software were used to estimate the sizes of DNA fr agments based on their mobilities relative to in-lane size markers. Th is allowed confident identification and comparison of FRAPD markers bo th within and between polyacrylamide gels. In comparison with analysis of RAPD products using ethidium bromide-stained agarose gels, fluores cent analysis Improved the sensitivity, resolution and precision of si zing of RAPD products of about 50-2100 bp. FRAPD fragments produced fr om amplification of zebrafish DNA are informative as genetic markers t hat segregate with Mendelian inheritance. FRAPD analysis was found to be very efficient for identifying new DNA polymorphisms.