S. Alkan et al., Usefulness of molecular detection of human herpesvirus-8 in the diagnosis of Kaposi sarcoma by fine-needle aspiration, AM J CLIN P, 111(1), 1999, pp. 91-96
Citations number
31
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Kaposi sarcoma (KS), a multifocal angioproliferative disorder occurs most c
ommonly in patients with AIDS, in whom it remains an important cause of mor
bidity and mortality. KS is often in the differential diagnosis in HIV-infe
cted patients undergoing fine-needle aspiration (FNA). FNA diagnosis of KS
is usually made by morphologic observation of scant tissue fragments compos
ed of bland spindle cells and crush artifact Human herpesvirus-8 (HHV-8) ha
s been identified by polymerase chain reaction (PCR) amplification of DNA s
amples isolated from various epidemiologic forms of KS. In apr attempt to i
mprove the accuracy of KS diagnosis by FNA, we analyzed for the presence of
HHV-8 DNA in 13 spindle-cell lesions evaluated by FNA: KS, 8 cases; granul
omatous inflammation due to Mycobacterium avium-intracellulare, 1; nodular
fasciitis, 1; dermatofibrosarcoma protuberans, 1; dermatofibroma, 1; benign
spindle-cell lesion of nerve sheath origin, 1; and 2 lesions with lymphoid
hyperplasia. DNA isolated from archival Wright-Giemsa-stained glass slides
was used for the PCR amplification of the HHV-8 DNA sequences. All of the
cases diagnosed as KS and I of the lymphoid hyperplasia cases were PCR-posi
tive for HHV-8 DNA, while all other cases of spindle-cell lesions were nega
tive. The molecular demonstration of HHV-8 DNA may be ct useful adjunct in
the diagnosis of KS by FNA.