Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds

Authors
Kostia, S Kantanen, J Kolkkala, M Varvio, SL
Citation
S. Kostia et al., Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds, ANIM GENET, 29(6), 1998, pp. 453-455
Citations number
5
Categorie Soggetti
Animal Sciences","Molecular Biology & Genetics
Journal title
ANIMAL GENETICS
ISSN journal
02689146 → ACNP
Volume
29
Issue
6
Year of publication
1998
Pages
453 - 455
Database
ISI
SICI code
0268-9146(199812)29:6<453:AOSAFM>2.0.ZU;2-L
Abstract
The applicability of single strand conformation polymorphism (SSCP) analysi s for major histocompatibility complex (MHC) genotyping in sheep was studie d. A panel of Ovar-DRB1 exon 2 'allele fingerprints' was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing seque nces are used as references in SSCP analysis. In this study, seven new exon 2 sequences and 19 different alleles in total were detected from 31 animal s of Finnish and Russian sheep breeds. Ovar-DRB1*0201 was detected in all t he six grey Finnsheep animals included in this study, suggesting reduced MH C diversity within these animals.