cDNA of eight nuclear encoded subunits of NADH : ubiquinone oxidoreductase: Human complex I cDNA characterization completed

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated mult iprotein complex located in the inner mitochondrial membrane. Its main func tion is the transport of electrons from NADH to ubiquinone, which is accomp anied by translocation of protons from the mitochondrial matrix to the inte rmembrane space. Human complex I appears to consist of 41 subunits of which 34 are encoded by nDNA. Here we report the cDNA sequences of the hitherto uncharacterized 8 nuclear encoded subunits, all located within the hydropho bic protein (HP) fraction of complex I. Now all currently known 41 proteins of human NADH:ubiquinone oxidoreductase have been characterized and report ed in literature, which enables more complete mutational analysis studies o f isolated complex I-deficient patients. (C) 1998 Academic Press.