Analysis of the ntp1(+) gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe

Authors
Soto, T Fernandez, J Dominguez, A Vicente-Soler, J Cansado, J Gacto, M
Citation
T. Soto et al., Analysis of the ntp1(+) gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe, BBA-GENE ST, 1443(1-2), 1998, pp. 225-229
Citations number
26
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1443
Issue
1-2
Year of publication
1998
Pages
225 - 229
Database
ISI
SICI code
0167-4781(19981126)1443:1-2<225:AOTNGE>2.0.ZU;2-3
Abstract
We have cloned and sequenced the ntp1(+) gene that codes for neutral trehal ase in the fission yeast Schizosaccharomyces pombe. The ntp1(+) gene produc t (Ntp1p) showed a 45-55% identity with neutral trehalases from other yeast s at the amino acid sequence level. However, in clear contrast to other neu tral yeast trehalases so far characterized (which show two cAMP phospho-sit es), only one consensus site for cAMP-dependent protein phosphorylation was found in Ntp1p. Northern blot hybridization experiments demonstrated that the Wis-Phh1/Sty1 MAP kinase cascade regulates ntp1(+) expression during os mostress. (C) 1998 Elsevier Science B.V. All rights reserved.