cDNA sequence analysis and expression of four long neurotoxin homologues from Naja naja atra

Authors
Qian, YC Fan, CY Gong, Y Yang, SL
Citation
Yc. Qian et al., cDNA sequence analysis and expression of four long neurotoxin homologues from Naja naja atra, BBA-GENE ST, 1443(1-2), 1998, pp. 233-238
Citations number
18
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1443
Issue
1-2
Year of publication
1998
Pages
233 - 238
Database
ISI
SICI code
0167-4781(19981126)1443:1-2<233:CSAAEO>2.0.ZU;2-I
Abstract
The novel cDNAs encoding four long neurotoxin homologues were firstly clone d from the venom gland of Naja naja atra by reverse transcriptase-polymeras e chain reaction. Amino acid sequence comparison showed that they may come from two different evolutionary origins. The mature proteins were expressed as soluble fusion proteins in Escherichia coli and purified for immunoblot ting, The results revealed that they displayed different immunochemical pro perties. (C) 1998 Elsevier Science B.V. All rights reserved.