Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor

Authors
Zhang, YL Spremulli, LL
Citation
Yl. Zhang et Ll. Spremulli, Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor, BBA-GENE ST, 1443(1-2), 1998, pp. 245-250
Citations number
16
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1443
Issue
1-2
Year of publication
1998
Pages
245 - 250
Database
ISI
SICI code
0167-4781(19981126)1443:1-2<245:IACOHM>2.0.ZU;2-K
Abstract
The complete sequence of the cDNA for the coding region of human mitochondr ial translational release factor 1 has been obtained from human EST clones and 5'RACE. This sequence has been analyzed to provide insights into the re lationship between release factors from bacteria and from mammalian mitocho ndria. The complete cDNA for the human mitochondrial ribosome recycling fac tor has been assembled using EST clones. This factor has been expressed in Escherichia coli and purified as a His-tagged protein. (C) 1998 Elsevier Sc ience B.V. All rights reserved.