Two-dimensional crystals of reconstituted beta-subunits of the chaperonin TF55 from Sulfolobus shibatae

Authors
Koeck, PJB Kagawa, HK Ellis, MJ Hebert, H Trent, JD
Citation
Pjb. Koeck et al., Two-dimensional crystals of reconstituted beta-subunits of the chaperonin TF55 from Sulfolobus shibatae, BBA-PROT ST, 1429(1), 1998, pp. 40-44
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
ISSN journal
01674838 → ACNP
Volume
1429
Issue
1
Year of publication
1998
Pages
40 - 44
Database
ISI
SICI code
0167-4838(199812)1429:1<40:TCORBO>2.0.ZU;2-R
Abstract
We have obtained 2-dimensional crystals of the P-subunits of the chaperonin TF55 from Sulfolobus shibatae reconstituted into oligomers in the absence of alpha-subunits. The subunits form rings with 9-fold rotational symmetry which arrange themselves in a trigonal lattice. From electron micrographs o f negatively stained specimens we have calculated a projection map in plane group p312 showing the rings in top-view. (C) 1998 Elsevier Science B.V. A ll rights reserved.