S. Subramanian et Pr. Adiga, Identification and mapping of linear antigenic determinants of chicken riboflavin carrier protein, BBA-PROT ST, 1429(1), 1998, pp. 74-82
Citations number
26
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
All of the possible 212 overlapping octapeptides covering the amino acid se
quence of chicken egg white riboflavin carrier protein (RCP) was synthesize
d on polyethylene rods. To identify the peptides constituting the sequentia
l antigenic epitopes on chicken RCP, a PEPSCAN ELISA was employed where ant
ibodies raised against SDS-treated, reduced and carboxymethylated RCP (RCM-
RCP) in mice, rats (n = 4), rabbits and monkeys (n = 3) were used. By this
approach, we could identify five sequences on chicken RCP commonly recogniz
ed by all the antisera from these species which were spanning the residues
10-17, 42-4, 134-141, 172-179 and 200-207. Analysis of the data for each in
dividual antiserum indicated inter- and intra-species variations with N- an
d C-terminal shifts in peak reactivity. The present study offers clues for
development of potential immunocontraceptive vaccines. (C) 1998 Elsevier Sc
ience B.V. All rights reserved.