ISOLATION OF THE GENE CODING FOR ELONGATION FACTOR-1-ALPHA IN CRYPTOSPORIDIUM-PARVUM

Authors
BONAFONTE MT PRIEST JW GARMON D ARROWOOD MJ MEAD JR
Citation
Mt. Bonafonte et al., ISOLATION OF THE GENE CODING FOR ELONGATION FACTOR-1-ALPHA IN CRYPTOSPORIDIUM-PARVUM, Biochimica et biophysica acta, N. Gene structure and expression, 1351(3), 1997, pp. 256-260
Citations number
31
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1351
Issue
3
Year of publication
1997
Pages
256 - 260
Database
ISI
SICI code
0167-4781(1997)1351:3<256:IOTGCF>2.0.ZU;2-V
Abstract
A gene encoding for Cryptosporidium parvum (C. parvum) elongation fact or 1 alpha (EF-1 alpha) was isolated and sequenced from a cDNA express ion library. The recombinant protein cross-reacted with a monoclonal a ntibody that was raised to a sporozoite cell surface antigen. The gene encoded a 435 amino acid protein with a predicted molecular weight of 48.1 kDa. The predicted C. parvum EF-1 alpha protein sequence showed extensive homology with the EF-1 alpha proteins of other eukaryotic or ganisms and included three conserved sequence motifs implicated in GTP binding. (C) 1997 Elsevier Science B.V.