CC chemokine receptors, CCR-1 and CCR-3, are potentially involved in antigen-presenting cell function of human peripheral blood monocyte-derived dendritic cells

We examined the potential involvement of two CC chemokine receptors (CCRs), CCR-1 and CCR-3, in the functional activation of granulocyte-macrophage co lony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4)-generated human peripheral blood monocyte-derived immature dendritic cells (DCs). Flow cyto metric analysis showed that CCR-1, CCR-3, CCR-5, and CXC chemokine receptor (CXCR)-4 were expressed on the cell surface of monocyte-derived DCs. Treat ment with a monoclonal antibody (MoAb) to either CCR-1 or CCR-3 but not MoA bs to CCR-5 and CXCR-4 abolished chemotactic migration of monocyte-derived DCs. The DCs treated with either the anti-CCR-1 MoAb or anti-CCR-3 MoAb wer e less efficient than untreated DCs in proliferation of allogeneic T cells (TCs) and TC-derived secretion of interferon-gamma (IFN-gamma). The homotyp ic aggregation of DCs and heterotypic aggregation of DCs with TCs were supp ressed by the anti-CCR-1 MoAb or anti-CCR-3 MoAb. These results indicate th at CCR-1 and CCR-3 specifically regulate interaction of TCs and DCs in the process of antigen presentation. (C) 1999 by The American Society of Hemato logy.