Central region-of-interest analysis of lumbar spine densitometry demonstrates lower bone mass in older rhesus monkeys

Osteoarthritis (OA) spuriously elevates spine bone mineral density (BMD) as measured by dual-energy S-ray absorptiometry (DXA), This study documents s pinal Oh prevalence in adult female rhesus monkeys, and evaluates a custom central region-of-interest (CROI) analysis technique designed to minimize O A effects on BMD measurement. Lumbar spine radiographs were obtained on 71 animals, age 10-37 years. OA degree was blindly scored as none, minimal, or moderate/severe. Moderate/severe OA was not observed before age 19, but wa s present in 66% of older animals, Subsequently, lumbar spine (L2-4) BMD wa s determined by standard DXA analysis and manual placement of 0.92 cm(2) CR OIs in two groups of female rhesus monkeys, One group (eight control, eight postovariectomy, ages 10-19 years) was assessed longitudinally, the second (n = 90, ages 10-37) cross-sectionally. Measured bone loss following ovari ectomy (8.1% standard analysis, 11.5% CROI) was comparable with both techni ques, By contrast, CROI demonstrated low er bone mass with age (p < 0.0001) , whereas only a trend (p = 0.06) was observed with standard analysis, When World Health Organization criteria were applied, 42% of animals greater th an or equal to age 19 years were classified as osteopenic/osteoporotic by s tandard analysis compared with 67% by CROI, All "normal" animals reclassifi ed as osteopenic/osteoporotic by CROI had OA. In conclusion, female rhesus monkeys often develop spinal OA with advancing age. CROI analysis demonstra ted Il,n er bone density in older monkeys and was as sensitive to estrogen- depletion bone loss as standard methodology. This suggests that alternative analysis techniques, such as CROI, may be more appropriate to evaluate bon e density in nonhuman primates, acid potentially in people. (C) 1999 by Els evier Science Inc. rill rights reserved.