Reversible binding of ethacrynic acid to human serum albumin: Difference circular dichroism study

Authors
Bertucci, C Nanni, B Salvadori, P
Citation
C. Bertucci et al., Reversible binding of ethacrynic acid to human serum albumin: Difference circular dichroism study, CHIRALITY, 11(1), 1999, pp. 33-38
Citations number
31
Categorie Soggetti
Chemistry & Analysis
Journal title
CHIRALITY
ISSN journal
08990042 → ACNP
Volume
11
Issue
1
Year of publication
1999
Pages
33 - 38
Database
ISI
SICI code
0899-0042(1999)11:1<33:RBOEAT>2.0.ZU;2-F
Abstract
The reversible binding of ethacrynic acid was characterized by a difference circular dichroism method. A 2/1 stoichiometry was determined for the [dru g]/[HSA] (human serum albumin) complex. The reversible binding of ethacryni c acid to HSA determines direct competition with ligands that selectivity b ind to site II and to the fatty acid site. Furthermore, indirect competitio n was shown for ligands for site I (anti-cooperative) and to site III (coop erative). Chirality 11:33-38, 1999. (C) 1999 Wiley-Liss, Inc.