Cytoskeletal organization and incorporation of beta 3 integrin in thrombin-stimulated platelets: Effect of acetylsalicylic acid

Platelet stimulation by agonists is followed by changes in cytoskeletal org anization that includes actin polymerization and association of the membran e skeleton (which is connected with the integrin alpha IIb beta 3) with the underlying cytoplasmic actin filaments. The effect of orally administered acetylsalicylic acid to healthy volunteers on incorporation of contractile protein and beta 3 integrin into the cytoskeletal core of thrombin-stimulat ed platelets was studied. Stimulation was followed by increased contractile protein and beta 3 incorporation into the cytoskeleton. Acetylsalicylic ac id intake resulted in decreased incorporation of myosin and actin (32% and 20%, respectively), and a decrease (36%) in the association of beta 3 integ rin with the cytoskeletal elements was evident. In conclusion, we have show n that acetylsalicylic acid, besides the known inhibitory effect on thrombo xane synthesis, promotes changes in the cytoskeletal organization of thromb in-stimulated platelets that could limit thrombus formation.