Heat production and substrate oxidation in rats fed at maintenance level and during fasting

A total of 36 Wistar rats were fed a commercial diet to a stipulated live w eight of 75 g (Group A), 100 g (Group B) and 225 g (Group C). All rats were measured in energy balance experiments, in which the animals were fed near maintenance level, followed by a period of fasting with measurements of th e gas exchange. The rats in Group A, B and C were fasted for 2, 3 and 4 day s, respectively. The minimum heat production on the last day of fasting for all groups was proportional to metabolic body weight (kg(0.75)) with a reg ression: heat production, kJ day(-1) = 321 x kg(0.75) (R-2 = 0.994). In rat s fed near maintenance level, heat production was provided by oxidation of carbohydrates in 80-85%, oxidation of protein was 10-15%, while oxidation o f fat contributed less than 10%,. It is suggested that in the fasting perio d, the contribution to the total heat production front oxidized carbohydrat e and fat depended on the size of the fat depots, a large fat depot giving rise to fat or;oxidation. On the last day of fasting, 24, 51 and 90% of the total heat originated from fat oxidation in Group A, B and C, respectively . (C) 1998 Elsevier Science Inc. All rights reserved.