G. Schenk et al., MOLECULAR EVOLUTIONARY ANALYSIS OF THE THIAMINE-DIPHOSPHATE-DEPENDENTENZYME, TRANSKETOLASE, Journal of molecular evolution, 44(5), 1997, pp. 552-572
Members of the transketolase group of thiamine-diphosphate-dependent e
nzymes from 17 different organisms including mammals, yeast, bacteria,
and plants have been used for phylogenetic reconstruction. Alignment
of the amino acid and DNA sequences for 21 transketolase enzymes and o
ne putative transketolase reveals a number of highly conserved regions
and invariant residues that are of predicted importance for enzyme ac
tivity, based on the crystal structure of yeast transketolase. One par
ticular sequence of 36 residues has some similarities to the nucleotid
e-binding motif and we designate it as the transketolase motif. We rep
ort further evidence that the recP protein from Streptococcus pneumoni
ae might be a transketolase and we list a number of invariant residues
which might be involved in substrate binding. Phylogenies derived fro
m the nucleotide and the amino acid sequences by various methods show
a conventional clustering for mammalian, plant, and gramnegative bacte
rial transketolases, The branching order of the gram-positive bacteria
could not be inferred reliably. The formaldehyde transketolase (somet
imes known as dihydroxyacetone synthase) of the yeast Hansenula polymo
rpha appears to be orthologous to the mammalian enzymes but paralogous
to the other yeast transketolases. The occurrence of more than one tr
ansketolase gene in some organisms is consistent with several gene dup
lications. The high degree of similarity in functionally important res
idues and the fact that the same kinetic mechanism is applicable to al
l characterized transketolase enzymes is consistent with the propositi
on that they are all derived from one common ancestral gene. Transketo
lase appears to be an ancient enzyme that has evolved slowly and might
serve as a model for a molecular clock, at least within the mammalian
clade.