Background: Adamantiades-Behcet's disease is a chronic systemic disorder as
sociating oral and genital ulcerative lesions with ocular and cutaneous man
ifestations, Previous publications report increased superoxide production b
y neutrophils and macrophages, increases in cytokines and malondialdehyde (
MDA), as well as low levels of enzymatic antioxidant defenses. Aim: We look
ed for another marker of oxidative stress in Adamantiades-Behcet's disease:
the presence of clastogenic factors (CF) in patients' plasma. In addition,
we determined plasma endproducts of lipid peroxidation (MDA). Patients and
Methods: We studied 20 patients and 20 controls. The clastogenic activity
was evaluated by means of cytogenetic methods. This test (CF test) detects
circulating prooxidants, due to their clastogenic effects after exposure of
lymphocyte cultures of healthy persons to plasma ultrafiltrates from patie
nts. The clastogenic prooxidants are lipid peroxidation products and cytoki
nes, in particular TNF-alpha. Lipid peroxidation was evaluated by the Ya,oi
method. Results: The CF test was positive in 18 out of 20 patients, while
it was negative in all 20 control persons. The mean increase in chromosomal
breaks was 10.6+/-3.8 in cultures exposed to patients' plasma and 1.3+/-2.
4 for cultures receiving control plasma (p<0.001). The clastogenic effect o
f patients' plasma ultrafiltrates was significantly inhibited by superoxide
dismutase (EC 1.15.1.1), suggesting an important role of the superoxide ra
dical in the clastogenic pathway. Thiobarbituric-acid-reactive substances (
expressed as nanomoles MDA per milliliter) were also significantly increase
d in these patients: 10.6+/-3.2 for patients and 6.6+/-1.4 for controls (p<
0.001). Conclusion: The presence of CF in the plasma of patients, indicatin
g the presence of circulating prooxidants with chromosome-damaging effects,
confirms an oxidative stress in Adamantiades-Behcet's disease. The anticla
stogenic effect of superoxide dismutase in vitro suggests the implication o
f the superoxide radical. MDA levels were also significantly increased in p
atients.