Mg. Quintana et H. Dalton, Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads, ENZYME MICR, 24(3-4), 1999, pp. 232-236
In the present study, we have investigated the biotransformation of differe
nt aromatic compounds to their cis-dihydrodiols (cis-diols) with immobilize
d Pseudomonas putida UV4 cells using barium alginate as the immobilization
medium for the cells in a fluidized-bed reactor. The reactions have been pe
rformed in a biphasic system containing 20% (v/v) tetradecane. The bacteria
l beads were fluidized using a flow of pure oxygen at a flow rate of tao mi
min(-1). The production of toluene cis-diol under the same conditions by t
he recombinant organism Escherichia coli JM109 (pKST11) was compared. The p
roduction of toluene cis-diol with E. coli JM109 (pKST11) was more than 24
times higher that the values reported in the literature using similar recom
binant non-immobilized organisms. The capacity of immobilized E. coli JM109
(pKST11 to yield cis-diols from different aromatic substrates in small-sca
le biotransformations was also investigated. (C) 1998 Elsevier Science Inc.