Full-term delivery following intracytoplasmic sperm injection with frozen-thawed immotile testicular spermatozoa

We present one of the very few deliveries occurring following intracytoplas mic sperm injection of thawed immotile testicular spermatozoa from a testic ular biopsy of a man with bilateral congenital absence of the vas deferens, A. first attempt in the 33 year old woman with fresh testicular biopsy ext racted spermatozoa was unsuccessful. The supernumerary spermatozoa were cry opreserved for later use. After thawing, testicular spermatozoa were immoti le. From 11 intact oocytes injected with frozen-thawed immotile testicular spermatozoa, a two pronuclear fertilization rate of 27% and a cleavage rate of 100% were obtained, A total of three embryos was transferred resulting in a singleton pregnancy and the birth of a normal female baby.