P-31 NMR IDENTIFICATION OF METABOLITES AND PH DETERMINATION IN THE CYANOBACTERIUM SYNECHOCYSTIS SP PCC-6308

Authors
LAWRENCE BA POLSE J DEPINA A ALLEN MM KOLODNY NH
Citation
Ba. Lawrence et al., P-31 NMR IDENTIFICATION OF METABOLITES AND PH DETERMINATION IN THE CYANOBACTERIUM SYNECHOCYSTIS SP PCC-6308, Current microbiology, 34(5), 1997, pp. 280-283
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Current microbiology → ACNP
ISSN journal
03438651
Volume
34
Issue
5
Year of publication
1997
Pages
280 - 283
Database
ISI
SICI code
0343-8651(1997)34:5<280:PNIOMA>2.0.ZU;2-T
Abstract
The identity of a number of phosphorus-containing metabolites present in Synechocystis sp. PCC 6308 has been confirmed by P-31 NMR spectrosc opy. The presence of D-ribulose 1,5-bisphosphate (RuBP); DL-glyceralde hyde 3-phosphate (GlyP); D(-) 3-phosphoglyceric acid (3PGA); D-ribulos e 5-phosphate (Ru5P); 6-phosphogluconic acid (6PGA); phosphoenolpyruva te (PEP); inorganic phosphate (Pi); uridine diphosphoglucose (UDPG); A DP and ATP were demonstrated by the pH dependence of their P-31 NMR ch emical shifts in spectra of perchloric acid cell extracts. Intracellul ar pH of cells was determined to be 7.5-7.7.