Identification and estimation of endogenous digoxin in biological fluids and tissues by TLC and HPLC

Authors
Arun, P Ravikumar, A Leelamma, S Kurup, PA
Citation
P. Arun et al., Identification and estimation of endogenous digoxin in biological fluids and tissues by TLC and HPLC, I J BIOCH B, 35(5), 1998, pp. 308-312
Citations number
11
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
ISSN journal
03011208 → ACNP
Volume
35
Issue
5
Year of publication
1998
Pages
308 - 312
Database
ISI
SICI code
0301-1208(199810)35:5<308:IAEOED>2.0.ZU;2-K
Abstract
A procedure for estimation of digoxin in biological samples after adding a known quantity of digoxin followed by extraction, separation by TLC and HPL C is, described The identity of digoxin thus extracted from rat brain has b eat established by reaction with digoxin antibody and by its inhibition of Na+-K+ ATPase activity. The method could be a better substitute to the rout ine radioimmunoassay as interfering substances are removed by TLC and HPLC.