Campylobacter jejuni-stimulated secretion of interleukin-8 by INT407 cells

Incubation of INTJ07 cells with various clinical isolates of Campylobacter jejuni resulted in secretion of interleukin-8 (IL-8) at levels ranging from 96 to 554 pg/ml at 21 h, The strains which produced the highest levels of Its secretion were 81-176 and BT44. Induction of IL-8 secretion required li ve cells of 81-176 and was dependent on de novo protein synthesis, Site-spe cific mutants of 81-176, which were previously shown to be defective in adh erence and invasion, resulted in reduced levels of secretion of IL-8, and c heY mutants of strains 81-176 and 749, which are hyperadherent and hyperinv asive, resulted in higher levels of IL-8 secretion. Another mutant of 81-17 6, which adheres at about 43% of the wild-type levels but is noninvasive, a lso showed marked reduction in LL-g levels, suggesting that invasion is nec essary for high levels of IL-8 secretion. When gentamicin was added to INT4 07 cells at 2 h after infection with 81-176, IL-8 secretion 22 h later was equivalent to that of controls without gentamicin, suggesting that the even ts which trigger induction and release of IL-8 occur early in the interacti ons of bacteria and eukaryotic cells.