Identification and functional characterization of the Neisseria gonorrhoeae lbpB gene product

Authors
Biswas, GD Anderson, JE Chen, CJ Cornelissen, CN Sparling, PF
Citation
Gd. Biswas et al., Identification and functional characterization of the Neisseria gonorrhoeae lbpB gene product, INFEC IMMUN, 67(1), 1999, pp. 455-459
Citations number
33
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
67
Issue
1
Year of publication
1999
Pages
455 - 459
Database
ISI
SICI code
0019-9567(199901)67:1<455:IAFCOT>2.0.ZU;2-N
Abstract
We cloned lbpB, encoding a predicted 80-kDa lipoprotein, upstream of lbpA. A nonpolar mutant (LbpB(-) LbpA(+)) had normal lactoferrin (LF) binding and grew normally with LF as an iron source, whereas LbpB(-) LbpA(-) and LbpB( +) LbpA(-) strains had reduced binding of LF and did not grow with LF as an iron source. LbpB bound LF directly in an affinity purification, suggestin g that LbpB might play a still-uncharacterized role in the LF iron utilizat ion.