Sequence heterogeneity in a repetitive DNA element of Fasciola

Citation
F. Kramer et T. Schnieder, Sequence heterogeneity in a repetitive DNA element of Fasciola, INT J PARAS, 28(12), 1998, pp. 1923-1929
Citations number
16
Categorie Soggetti
Biology,Microbiology
Journal title
INTERNATIONAL JOURNAL FOR PARASITOLOGY
ISSN journal
00207519 → ACNP
Volume
28
Issue
12
Year of publication
1998
Pages
1923 - 1929
Database
ISI
SICI code
0020-7519(199812)28:12<1923:SHIARD>2.0.ZU;2-G
Abstract
A repetitive DNA sequence used as a specific probe for Fasciola hepatica in fections in snails was examined in F. hepatica and Fasciola gigantica (36 i ndividuals) specimens from five continents. The degree of intraspecific ide ntity ranged from 79 to 99% in F. hepatica and from 93 to 99% in F. giganti ca. The interspecific identity ranged from 81 to 100%, confirming the sugge stion that the DNA probe sequence could be used worldwide as an epidemiolog ical tool for the examination of the intermediate host snail in fasciolosis . Differentiation between F. hepatica and F. gigantica infections in snails was not possible using the probe. (C) 1998 Australian Society for Parasito logy. Published by Elsevier Science Ltd. All rights reserved.