Determination of genital human papillomavirus genotypes in women in Northern Australia using a novel, self-administered tampon technique

The age standardized death rate from cervical cancer in Aboriginal women in the Northern Territory (NT) for the period 1987 to 1993 was 11.5 times hig her than the Australian average for the same period. This is the first stud y to determine HPV genotypes in both Aboriginal and non-Aboriginal women li ving in the Top End of the NT using a self-administered tampon technique fo r specimen collection. Women who attended sexually transmitted disease clin ics and Family Planning Clinics in urban areas and community health centers in remote areas were asked to insert and immediately remove a tampon which was then tested for the presence of HPV genotypes using the polymerase cha in reaction. A total of 646 female subjects were enrolled in the study. Sub jects with HPV had a mean age of 26.1 years (SD 8.5), while those without H PV had a mean age of 29.8 years (SD 9.8) (P < 0.001). The oncogenic genotyp es (16,18,31,33,35,39,45,51,52) accounted for 54.7%, while 23.1% of HPVs we re untypable. The virus was detected in 161/287 (56.1%) of non-Aboriginal w omen and in 150/359 (41.8%) of Aboriginal women (P < 0.001). More than one genotype was detected in 40 subjects (12.8%); of these seven had three or m ore genotypes present. This study is the first to document the HPV genotype s occurring in females in the Northern Territory and shows that HPV is a co mmon infection in both the Aboriginal and non-Aboriginal population.