A method for rapid analysis of pesticides causing acute poisoning in patients and application of this method to clinical treatment

Pesticide concentration in serum must be measured in order to determine the proper treatment for patients with pesticide poisoning. We previously investigated the effectiveness of a screening method for fat- soluble pesticides by HPLC equipped with photo-diode-array detection (HPLC- DAD) and found that the method was effective in emergency medical units. In the present study, we investigated the effectiveness of a method for qua ntitative analysis for these fat-soluble pesticides. The concentrations of all pesticides were proportional to the peak area up to 100 mu g/ml (injection amount: 1 mu g), and the recovery ratio was excel lent. This method was applied to three actual cases of acute poisoning, and we we re able to give important advice to the doctor according to the concentrati on of the pesticides in the serum. The screening of pesticides by HPLC-DAD was confirmed by GC-MS.