DEVELOPMENT OF LUMINESCENT TERBIUM(III) CHELATES FOR PROTEIN LABELING- EFFECT OF TRIPLET-STATE ENERGY-LEVEL

Authors
TAKALO H MUKKALA VM MERIO L RODRIGUEZUBIS JC SEDANO R JUANES O BRUNET E
Citation
H. Takalo et al., DEVELOPMENT OF LUMINESCENT TERBIUM(III) CHELATES FOR PROTEIN LABELING- EFFECT OF TRIPLET-STATE ENERGY-LEVEL, Helvetica Chimica Acta, 80(2), 1997, pp. 372-387
Citations number
41
Categorie Soggetti
Chemistry
Journal title
Helvetica Chimica Acta → ACNP
ISSN journal
0018019X
Volume
80
Issue
2
Year of publication
1997
Pages
372 - 387
Database
ISI
SICI code
0018-019X(1997)80:2<372:DOLTCF>2.0.ZU;2-Q
Abstract
The synthesis of novel Tb-III labels suitable for protein labelling ar e reported. Their luminescence properties as antibody conjugates were measured and compared to the results of corresponding Tb-III chelates of the parent ligand structures. When the lowest triplet-state energy level of the parent donor ligand was over 23 000 cm(-1), i. e., the en ergy gap between the D-5(4) level of Tb-III and the lowest triplet-sta te energy level of the ligand exceeded 2600 cm(-1), the label derivati ve with a long decay time (tau=1.35-2.93 ms) and a high luminescence y ield (epsilon.Phi=3770-4560) was found to be suitable for bioaffinity assays.