Synthesis and characterization of a polymer-coated C-18 stationary phase with high carbon content for liquid chromatography

High-purity porous silica with a large surface area (particle diameter 5 mu m, mean pore diameter 8.4 nm, surface area 350 m(2)/g) was coated with a h omogeneous silicone polymer film, which was thereafter modified with octade cyl groups to be used as a stationary phase for reversed-phase chromatograp hy. The stationary phase had a high carbon content (18%), and showed a rela tively large overall retention for various analytes among other stationary phases available in the market. It also showed preferable characteristics i n separation of basic compounds, such as pyridine, and hinokitiol, a substa nce that easily coordinates with metals, and also had a high durability und er an alkaline mobile phase. (C) 1998 Elsevier Science B.V. All rights rese rved.