Structural characteristics of low-molecular-mass displacers for cation-exchange chromatography - II. Role of the stationary phase

The relative efficacy of a variety of low-molecular-mass displacers was exa mined on three different stationary phase materials. Several homologous ser ies of displacer molecules were evaluated on these ion-exchange resins usin g a displacer ranking plot based on the steric mass action model. The resul ts demonstrate that while aromaticity and hydrophobicity can play a signifi cant role in the affinity of displacer molecules on polymethacrylate based and hydrophilized polystyrene-divinylbenzene based materials, this effect i s much less pronounced on an agarose based resin. The work presented in thi s paper demonstrates that different structural features of low-molecular-ma ss displacers can dominate their affinity on various stationary phase mater ials employed and provides rules of thumb for the design of high affinity, low-molecular-mass displacers for a variety of commercial cation-exchange m aterials. (C) 1998 Published by Elsevier Science B.V. All rights reserved.