Rl. Fahrner et al., Real-time control of purified product collection during chromatography of recombinant human insulin-like growth factor-I using an on-line assay, J CHROMAT A, 827(1), 1998, pp. 37-43
During preparative reversed-phase chromatography of recombinant human insul
in-like growth factor-I (IGF), the separation of IGF from IGF aggregates ca
nnot be determined using UV absorbance. An on-line reversed-phase chromatog
raphic assay was developed that provides a quantitative measurement of ICF
and IGF aggregates every 4 min, allowing real-time control of purified IGF
collection. Process control using the on-line assay is a reliable and accur
ate method to collect purified IGF. (C) 1998 Elsevier Science B.V. All righ
ts reserved.