The novel histamine H-2 receptor antagonist FRG-8813 prevents delay of wound repair induced by hydrogen peroxide in a rabbit gastric epithelial cell system

The effects of a novel histamine H-2 receptor antagonist (FRG-8813) on the restoration process of gastric epithelial wounds were assessed using an in vitro wound healing model. FRG-8813 (1, 10 mol/L) was added to a complete c onfluent monolayer cell sheet after artificial wounding. The restoration pr ocess was analysed by a time-lapse video system and cell migration, prolife ration and apoptosis were assessed. Hydrogen peroxide (1, 3 mmol/L) inhibit ed restoration after wounding by suppressing cell migration and proliferati on and induced epithelial cell apoptosis around the wound. The addition of FRG-8813 abolished the hydrogen peroxide-induced retardation and prevented apoptosis, although FRG-8813 itself did not enhance wound healing. FRG-8813 may act as a radical scavenger as well as having an anti-secretory action and may have favourable effects on peptic ulcer healing.