Np. O'Grady et al., Detection of macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta during experimental endotoxemia and human sepsis, J INFEC DIS, 179(1), 1999, pp. 136-141
Macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta regulate leuko
cyte activation and trafficking, To assess the role of MIP-1 alpha and MIP-
1 beta in human inflammation, healthy subjects were studied during experime
ntal endotoxemia with prior administration of ibuprofen, a cyclooxygenase i
nhibitor, or dimeric p75 tumor necrosis factor (TNF)-alpha receptor, a TNF
antagonist; septic patients were also studied. Following endotoxin, blood l
evels of both MIP-1 molecules rose acutely and fell to baseline by 6 h (P =
.001). While MIP-1 mediates fever in animals independent of cyclooxygenase
blockade, in subjects given endotoxin and ibuprofen, MIP-1 levels increase
d and fever was suppressed, MIP-1 levels were not diminished by inhibiting
circulating TNF-alpha in humans. In septic patients, elevated levels of MIP
-1 alpha and MIP-1 beta were detected within 24 h of sepsis and fell in par
allel with TNF-alpha and interleukin-6 (P < .01). MIP-1 alpha and MIP-1 bet
a increase during acute inflammation but are not associated with fever in e
ndotoxemic humans during cyclooxygenase blockade.