Rapid discrimination of psychrotolerant and mesophilic strains of the Bacillus cereus group by PCR targeting of 16S rDNA

Authors
von Stetten, F Francis, KP Lechner, S Neuhaus, K Scherer, S
Citation
F. Von Stetten et al., Rapid discrimination of psychrotolerant and mesophilic strains of the Bacillus cereus group by PCR targeting of 16S rDNA, J MICROB M, 34(2), 1998, pp. 99-106
Citations number
27
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
01677012 → ACNP
Volume
34
Issue
2
Year of publication
1998
Pages
99 - 106
Database
ISI
SICI code
0167-7012(199811)34:2<99:RDOPAM>2.0.ZU;2-7
Abstract
The paper describes a novel PCR assay for discriminating psychrotolerant an d mesophilic strains of the Bacillus cereus group by targeting of 16S rDNA signatures. Application of the assay circumvents long-term growth tests at low temperature currently used to detect psychrotolerant strains. PCR was p erformed with pure cultures. A 100% correlation of PCR and growth data at 7 degrees C was obtained for the 194 B. cereus group strains tested. Potenti al applications of the assay for the dairy industry and agriculture are sug gested. (C) 1998 Elsevier Science B.V. All rights reserved.