Distinction of the species of mushrooms by capillary electrophoresis

Capillary electrophoresis (CE) has been applied to distinguish the species of mushrooms with similar shapes morphologically. Water-extracts of mushrooms were cleaned up with a Sep-Pak C-18 cartridge u sing methanol. After evaporation, the residue was dissolved in water and 50 mmol/L anhydrous caffeine was added as an internal standard for the analys is by CE. Migration time (MT) of electroosmotic flow (EOF), relative migrat ion (RM) and mobility (mu) on CE-grams were used as indexes for distinguish ing the species of mushrooms. Naematoloma sublateritium and Naematoloma fas ciculare, Lentinus edodes and Lampteromyces japonicus and Rhodophylus rhodo pholius and Rhodophyllus crassipes could be distinguished by this method. Indexes of numerical values of RM and mu on CE-grams were species-specific, indicating that mushroom species might be identified by analyzing the wate r-soluble proteins of mushroom.