An analytical method by GC/MS has been established for the herbicide difenz
oquat in cereals. Difenzoquat was extracted with acetone, concentrated with
a rotary evaporator, and applied to an Extrelut 20 column. The column was
washed with n-hexane, eluted with ethyl acetate containing 4% formic acid,
and concentrated. The residue was dissolved in 0.1 mol/L NaOH solution, ads
orbed on an ODS mini column, washed with 50% methanol, and eluted with ethy
l acetate containing 4% formic acid. The elute was concentrated and analyze
d by GC/MS. This method gave a recovery of over 80% with a C.V. below 6% an
d a detection limit of 1 mu g/kg. This method, with excellent sensitivity a
nd selectivity, should be useful as a routine analytical method for cereals
.