Flow cytometric analysis for enterotoxin exposed on Clostridium perfringens spores

Authors
Kusunoki, H Hu, D Piyankarage, RH Sugii, S Uemura, T
Citation
H. Kusunoki et al., Flow cytometric analysis for enterotoxin exposed on Clostridium perfringens spores, J VET MED S, 60(12), 1998, pp. 1357-1359
Citations number
23
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN journal
09167250 → ACNP
Volume
60
Issue
12
Year of publication
1998
Pages
1357 - 1359
Database
ISI
SICI code
0916-7250(199812)60:12<1357:FCAFEE>2.0.ZU;2-T
Abstract
Flow cytometric method (FCM) with fluorescent-labeled anti-CPE antibody was applied to develop a rapid, specific, and convenient method to detect ente rotoxin (CPE) exposed on the surface of spores of Clostridium perfringens. The results obtained indicate that FCM can specifically detect CPE exposed on C. perfringens spores for a short rime. Thus, FCM is found to be a rapid , specific, and convenient assay method for detection of CPE exposed on C. perfringens spores, suggesting that it will be hopefully useful to diagnose food poisoning caused by C. perfringens.