Jz. Guo et al., Effects of a tachykinin NK3 receptor antagonist, SR 142801, studied in isolated neonatal rat spinal cord, NEUROPEPTID, 32(6), 1998, pp. 537-542
Effects of a nonpeptide tachykinin NK3 receptor antagonist, SR 142801,were
studied in the isolated spinal cord preparation of the neonatal rat. Potent
ial changes were recorded extracellularly from a lumbar ventral root. Bath-
application of neurokinin B induced a dose-dependent depolarization of the
ventral root. SR 142801 caused rightward shifts of the concentration-respon
se curve for neurokinin B with pA(2) of 6.57, but did not affect the depola
rizing responses to other agonists. Stimulation of a dorsal root evoked in
the ipsilateral ventral root of the same segment monosynaptic and polysynap
tic reflexes of fast time course which were followed by a slow depolarizati
on (ipsilateral slow ventral root potential). SR 142801 depressed the ipsil
ateral slow ventral root potential. The present results indicate that SR 14
2801 is a specific antagonist for tachykinin NK, receptors in the spinal co
rd and suggest that NK, receptors are involved in primary afferent-evoked n
ociceptive responses of spinal neurones.