Chiral separation of enantiomers of amino acid derivatives by HPLC on vancomycin and teicoplanin chiral stationary phases

The chiral separation of alpha-amino acids by liquid chromatography using m acrocyclic antibiotic bonded stationary phases were studied. Teicoplanin an d vancomycin bonded stationary phases have been used to separate enantiomer s of dansyl amino acids in the reversed - phase high performance liquid chr omatography mode. By comparison of chromatographic parameters obtained by u se of both chiral stationary phases it the mechanism of chiral separation c ould be suggested. The better separation - greater value of R-j,R-i of enan tiomers - was achieved by the teicoplanin column.