Green fluorescent protein purification by organic extraction

Green fluorescent protein (GFP) is widely used as an excellent reporter mol ecule in biochemistry and cell biology. Some biochemical and immunological assays require high-purity GFP. However, the majority of current procedures for GFP purification include multiple time-consuming chromatography steps with a low yield of the desired product or require tag-containing proteins. An alternative method is described for the GFP purification without affini ty extensions using organic extraction yielding a highly homogeneous protei n indistinguishable in spectroscopic properties from that purified by previ ous methods. (C) 1998 Academic Press.