R. Weltzin et al., NOVEL INTRANASAL IMMUNIZATION TECHNIQUES FOR ANTIBODY INDUCTION AND PROTECTION OF MICE AGAINST GASTRIC HELICOBACTER-FELIS INFECTION, Vaccine, 15(4), 1997, pp. 370-376
Intranasal (i.n.) delivery of antigen can be highly effective for gene
rating circulating and secretory antibody responses. Mice were immuniz
ed i.n. with two antigens, human IgA, and Helicobacter pylori urease i
n the presence or absence of mucosal adjuvant. To restrict antigen del
ivery to the upper airways, protein solutions were administered in a s
mall volume without anesthesia. Repeated daily i.n. administration of
antigen without adjuvant elicited high levels of specific IgG in serum
and IgA in serum, saliva, and feces. Once weekly i.n. immunization wi
th co-administration of cholera toxin or Escherichia coli heat-labile
toxin as adjuvant elicited somewhat lower levels of antibody to urease
. When challenged with Helicobacter felis, only mice immunized with ur
ease in the presence of adjuvant were protected against gastric infect
ion. (C) 1997 Elsevier Science Ltd.