K. Kamaike et al., Oligonucleotide synthesis using the 2-(levulinyloxymethyl)-5-nitrobenzoyl group for the 5 '-position of nucleoside 3 '-phosphoramidite derivatives, ACT BIOCH P, 45(4), 1998, pp. 949-976
A comparative study on the utility of 2-(levulinyloxymethyl)-5-nitrobenzoyl
(LMNBz) and 2-(levulinyloxymethyl)benzoyl (LMBz) protecting groups for the
5'-positions of nucleoside 3'-phosphoramidite derivatives in the oligonucl
eotide synthesis is presented in terms of the syntheses of TpTpT, TpTpTpT,
and UpCpApGpUpUpGpG. In addition we describe the synthesis, using the LMNBz
protecting group of the CpCpA terminus triplet of tRNAs bearing exocyclic
amino groups with N-15-labeling, and the trimer Gp[A*]pG containing 2'-O-(b
eta-D-ribofuranosyl)adenosine ([A*]), the latter of which is found at posit
ion 64 in the yeast initiator tRNA(Met).