Functional role of rRNAs in plant translation system tested with antisensestrategy

There are regions in rRNA which are evolutionary conserved and exposed on r ibosomal surface. We selected in plant material (Lupinus luteus) two of the m: the alpha-sarcin domain of 26S rRNA (L-rRNA) and C loop of 5S rRNA, to b e further investigated using antisense oligomers as research tools. We foun d inhibition of the model polypeptide biosynthesis (up to 80%) due to speci fic hybridization of oligomers addressed to a-sarcin domain and loop C. Bas ed on our results we present the evidence for the key role played by these regions of rRNAs during protein biosynthesis in plant system. According to our hypothesis, conformational changes of these two regions are synchronise d and cooperative during transition of pre- to post-translocation state of the ribosome. The correlation of structure and activity of rRNA domains in translation is shown.