Cyclic traction machine for long-term culture of fibroblast-populated collagen gels

Our research group has been investigating the effect of cyclic deformations on the evolution of fibroblast populated collagen gels (FPCG). Since exist ing traction machines are not designed for such an application, we had to d esign a cyclic traction machine adapted to tissue culture inside an incubat or over an extended period of time. Biocompatible materials were used for f abrication to allow for easy sterilization and to prevent any adverse react ion from the tissue. The traction machine is based on a computer-controlled stepping motor system for easy adjustment of the deformation amplitude and frequency. The maximum stretching speed achieved is around 1 mm/s. The tra ction machine can measure FPCG mechanical properties and perform rupture te sts to determine its ultimate strength. Several FPCGs have been successfull y cultured with the machine for up to four weeks without any adverse reacti on. (C) 1999 Biomedical Engineering Society. [S0090-6964(99)00401-4].