Rapid detection of Ophiostoma piceae and O-quercus in stained wood by PCR

Authors
Kim, SH Uzunovic, A Breuil, C
Citation
Sh. Kim et al., Rapid detection of Ophiostoma piceae and O-quercus in stained wood by PCR, APPL ENVIR, 65(1), 1999, pp. 287-290
Citations number
12
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
1
Year of publication
1999
Pages
287 - 290
Database
ISI
SICI code
0099-2240(199901)65:1<287:RDOOPA>2.0.ZU;2-1
Abstract
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived -specific primers, detection was feasible within 4 h, even with DNA obtaine d from a single synnema. This method can easily be extended for the detecti on of other wood-inhabiting fungi.