Interleukin 8 (IL-8) induces the expression of kinin B-1 receptor in humanlung fibroblasts

Kinin B-1 receptors are induced by various inflammatory mediators. The aim of the present study was to investigate the effect of the CXC chemokine IL- 8 on kinin B-1 receptor expression in IMR-90 cells, by performing binding s tudies and Northern blot analysis of B-1 receptor mRNA levels. We demonstra ted here that the density of the kinin B-1 receptors could be increased by the chemokine IL-8 in a concentration- and time-dependent manner. IL-8 also increased the kinin B-1 receptor mRNA level in IMR-90 cells. IL-8-induced B-1 receptor expression could be totally abolished by pretreatment with the metabolic inhibitors. Furthermore, expression was markedly reduced by anti bodies to human IL-1 beta. In conclusion, IL-8 increased the expression of kinin B-1 receptors in IMR-90 cells and this effect is likely to be seconda ry to the production of IL-1 beta. (C) 1998 Academic Press.