High-level expression of human interleukin-17 in the yeast Pichia pastoris

Authors
Zhou, LM Wang, JX Peng, SY Duan, JB Cai, X Zou, MJ Su, Q
Citation
Lm. Zhou et al., High-level expression of human interleukin-17 in the yeast Pichia pastoris, BIOC MOL B, 46(6), 1998, pp. 1109-1116
Citations number
10
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
ISSN journal
10399712 → ACNP
Volume
46
Issue
6
Year of publication
1998
Pages
1109 - 1116
Database
ISI
SICI code
1039-9712(199812)46:6<1109:HEOHII>2.0.ZU;2-R
Abstract
Human interleukin-17 (hIL-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by RT-PCR, then highly express ed in the yeast Pichia pastoris in the form of the glycosylated monomer. Th e monomer of rhIL-17 stimulated mouse fibroblast 3T3 cells to secrete IL-6 and was specifically bound to its receptors on 3T3 cells.